MersV1, Main, Exploration, bibRecord, 004E23

Immunological correspondence between arthropod hemocyanin subunits. I. Scorpion (Leiurus, Androctonus) and spider (Eurypelma, Cupiennius) hemocyanin.

Identifieur interne : 004E23 ( Main/Exploration ); précédent : 004E22; suivant : 004E24

Immunological correspondence between arthropod hemocyanin subunits. I. Scorpion (Leiurus, Androctonus) and spider (Eurypelma, Cupiennius) hemocyanin.

Auteurs : J. Markl ; W. Gebauer ; R. Runzler ; I. Avissar

Source :

Hoppe-Seyler's Zeitschrift fur physiologische Chemie [ 0018-4888 ] ; 1984.

RBID : pubmed:6479892

Descripteurs français

KwdFr :
- Animaux, Araignées (analyse), Fragments peptidiques (analyse), Hémolymphe (analyse), Immunochimie, Immunoélectrophorèse (), Réactions croisées, Scorpions (analyse), Spécificité d'espèce, Électrophorèse sur gel de polyacrylamide ().
MESH :
- analyse : Araignées, Fragments peptidiques, Hémolymphe, Scorpions.
- Animaux, Immunochimie, Immunoélectrophorèse, Réactions croisées, Spécificité d'espèce, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Animals, Cross Reactions, Electrophoresis, Polyacrylamide Gel (methods), Hemocyanins (analysis), Hemolymph (analysis), Immunochemistry, Immunoelectrophoresis (methods), Peptide Fragments (analysis), Scorpions (analysis), Species Specificity, Spiders (analysis).
MESH :
- chemical , analysis : Hemocyanins, Peptide Fragments.
- analysis : Hemolymph, Scorpions, Spiders.
- methods : Electrophoresis, Polyacrylamide Gel, Immunoelectrophoresis.
- Animals, Cross Reactions, Immunochemistry, Species Specificity.

Abstract

The hemocyanins of the scorpions Leiurus quinquestriatus and Androctonus australis, the tarantula Eurypelma californicum (all 24-mers), and the lycosid spider Cupiennius salei (dodecamer) were dissociated into subunits, the subunits isolated and studied by two-dimensional immunoelectrophoresis for interspecific cross-reactivities. Androctonus hemocyanin yielded a pattern of 8 subunit types in agreement with data from Lamy et al. (1979, Arch. Biochem. Biophys. 193, 140-149). Leiurus hemocyanin is also composed of 8 immunologically distinct subunits which could be assigned to the pattern of Androctonus in a subunit-to-subunit correlation. The subunit designations 1 to 6 of Lamy et al. could be adopted for both scorpion hemocyanins; however, in the present communication, Lamy's subunits 3A/3B are designated as 3'/3", because we could not unequivocally decide if 3' = 3A and 3" = 3B or vice versa. The 7 subunit types a to g of Eurypelma hemocyanin could be correlated with the scorpion hemocyanin subunits as follows: a = 3', b = 5B, c = 3C, d = 5A, e = 6, f = 2, g = 4. Additional cross-reactivities were detected between e/4, and f/5A, respectively. No subunit of Eurypelma hemocyanin is homologous to scorpion 3", which could not be precipitated by anti-Eurypelma antiserum. Antiserum against Cupiennius hemocyanin precipitated subunit f of Eurypelma and subunits 2 and 5A of scorpion hemocyanin. The published models of quaternary structure and a possible subunit phylogeny of arachnidan hemocyanins are discussed in view of the present results.

DOI: 10.1515/bchm2.1984.365.1.619
PubMed: 6479892

Affiliations:

Le document en format XML

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<term>Hemolymph (analysis)</term>
<term>Immunochemistry</term>
<term>Immunoelectrophoresis (methods)</term>
<term>Peptide Fragments (analysis)</term>
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<term>Araignées (analyse)</term>
<term>Fragments peptidiques (analyse)</term>
<term>Hémolymphe (analyse)</term>
<term>Immunochimie</term>
<term>Immunoélectrophorèse ()</term>
<term>Réactions croisées</term>
<term>Scorpions (analyse)</term>
<term>Spécificité d'espèce</term>
<term>Électrophorèse sur gel de polyacrylamide ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hemocyanins</term>
<term>Peptide Fragments</term>
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<term>Fragments peptidiques</term>
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<term>Scorpions</term>
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<term>Scorpions</term>
<term>Spiders</term>
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<term>Immunoelectrophoresis</term>
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<term>Cross Reactions</term>
<term>Immunochemistry</term>
<term>Species Specificity</term>
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<term>Immunochimie</term>
<term>Immunoélectrophorèse</term>
<term>Réactions croisées</term>
<term>Spécificité d'espèce</term>
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<front><div type="abstract" xml:lang="en">The hemocyanins of the scorpions Leiurus quinquestriatus and Androctonus australis, the tarantula Eurypelma californicum (all 24-mers), and the lycosid spider Cupiennius salei (dodecamer) were dissociated into subunits, the subunits isolated and studied by two-dimensional immunoelectrophoresis for interspecific cross-reactivities. Androctonus hemocyanin yielded a pattern of 8 subunit types in agreement with data from Lamy et al. (1979, Arch. Biochem. Biophys. 193, 140-149). Leiurus hemocyanin is also composed of 8 immunologically distinct subunits which could be assigned to the pattern of Androctonus in a subunit-to-subunit correlation. The subunit designations 1 to 6 of Lamy et al. could be adopted for both scorpion hemocyanins; however, in the present communication, Lamy's subunits 3A/3B are designated as 3'/3", because we could not unequivocally decide if 3' = 3A and 3" = 3B or vice versa. The 7 subunit types a to g of Eurypelma hemocyanin could be correlated with the scorpion hemocyanin subunits as follows: a = 3', b = 5B, c = 3C, d = 5A, e = 6, f = 2, g = 4. Additional cross-reactivities were detected between e/4, and f/5A, respectively. No subunit of Eurypelma hemocyanin is homologous to scorpion 3", which could not be precipitated by anti-Eurypelma antiserum. Antiserum against Cupiennius hemocyanin precipitated subunit f of Eurypelma and subunits 2 and 5A of scorpion hemocyanin. The published models of quaternary structure and a possible subunit phylogeny of arachnidan hemocyanins are discussed in view of the present results.</div>
</front>
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<tree><noCountry><name sortKey="Avissar, I" sort="Avissar, I" uniqKey="Avissar I" first="I" last="Avissar">I. Avissar</name>
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<name sortKey="Markl, J" sort="Markl, J" uniqKey="Markl J" first="J" last="Markl">J. Markl</name>
<name sortKey="Runzler, R" sort="Runzler, R" uniqKey="Runzler R" first="R" last="Runzler">R. Runzler</name>
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Immunological correspondence between arthropod hemocyanin subunits. I. Scorpion (Leiurus, Androctonus) and spider (Eurypelma, Cupiennius) hemocyanin.

Immunological correspondence between arthropod hemocyanin subunits. I. Scorpion (Leiurus, Androctonus) and spider (Eurypelma, Cupiennius) hemocyanin.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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